
To obtain the complete coding DNA sequence (CDS) for the two identified ESTs, we used RACE methods (GeneRacer Kit, Life Technologies/Thermo Fisher Scientific) with juvenile-squid total RNA and gene specific primers ().
CONSTRUCT SEMI CIRCLE ON GSP5 FULL
Then, create a rectangle shape and align it to cover the full circle. Rapid amplification of cDNA ends (RACE), cloning, and sequence analysis.

Method 2: Create a Semi Circle from a Full Circle using Merge Shapes feature in PowerPoint Draw a circle c and rotate the circle by 180 degrees about A to form the. You can further edit this partial shape by using Shape Format in PowerPoint to change the color or shape style, to add text to the shape and for making other types of adjustments, such as merging shapes. This transformation is the same as a half-turn, a rotation by 180 degrees. When you insert a Partial Shape, there is a small yellow dot available that lets you adjust the circle shape. The easiest way to draw a Semi Circle in PowerPoint is using the Partial Circle shape. Method 1: Create a Semi Circle from a Partial Circle Shape Dealing with data and observations has always been an important aspect of discovery in science. These include, circular diagrams, timelines, economic models, processes, marketing and sales presentations, etc. The first thing you need to do is figure out which runway you should land on. You can use semi circles in PowerPoint to construct diagrams for a variety of presentation topics. Similarly, semi circles are used in various other types of diagrams for creating models which can represent each part of the equation in detail.

Semi circles are used in an arithmetic and geometric mean, as well as in mechanical engineering. The semi circle always measures at 180°, with a single line of symmetry. Semi circles have had various applications in mathematics. What is a Semi Circle Diagram and When is it Used?
